IL-13 Ra2 ELISA KIT
INTENDED USE
This Human IL-13 Ra2 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 13 Rα2 concentrations in serum, plasma, cell culture supernatant. This IL-13 Ra2 ELISA Kit is intended FOR LABORATORY RESEARCH USE ONLY and not for use in diagnostic or therapeutic procedures.
PRINCIPLE OF THE ASSAY
IL-13 Ra2 ELISA Kit
IL-13 Ra2 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 13 Rα2 concentrations in serum, plasma, cell culture supernatant. This IL-13 Ra2 ELISA Kit is intended FOR LABORATORY RESEARCH USE ONLY and not for use in diagnostic or therapeutic procedures. This IL-13 Ra2 ELISA Kit applies the quantitative sandwich enzyme immunoassay technique.
A monoclonal antibody specific to IL-13 Rα2 has been pre-coated onto a microplate. Standards and samples are then added to the appropriate plate wells with a biotin -conjugated polyclonal antibody preparation specific for IL-13 Rα2 and incubated. IL-13 Rα2 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. After washing away any unbound substances. In order to quantitatively determine the amount of IL-13 Rα2 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) added to each well and incubated. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and only those wells that contain IL-13 Rα2, biotin conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The colour development is stopped and the intensity of the colour is measured.
In order to measure the concentration of IL-13 Rα2 in the samples, this package of reagent includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the IL-13 Ra2 ELISA Kit testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-13 Rα2 concentration (ng/mL). The concentration of IL-13 Rα2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Download Product Data Sheet