This human FSH ELISA kit applies a technique called a quantitative sandwich immunoassay. The minimal detectable concentration of human FSH by using this human FSH ELISA kit is estimated to be 1.5 mIU/mL. This human FSH ELISA kit exhibits no detectable cross-reaction with LH, HCG, TSH, and Prolactin. hGH can be detected in this assay.
Follicle stimulating hormone (FSH) is a glycoprotein. Each monomeric unit is a protein molecule with a sugar attached to it; two of these make the full, functional protein. Its structure is similar to LH, TSH, and hCG. The protein dimer contains 2 polypeptide units, labelled alpha and beta subunits. The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids. The beta subunits vary. FSH has a beta subunit of 118 amino acids (FSHB) that confers its specific biologic action and is responsible for interaction with the FSH-receptor. The sugar part of the hormone is composed of fucose, galactose, mannose, galactosamine, glucosamine, and sialic acid, the latter being critical for its biologic half-life. The half-life of FSH is 3-4 hours.
This human FSH ELISA kit is to be used for the in vitro quantitative determination of human follicle stimulating hormone (FSH) concentrations in serum. In order to measure the concentration of FSH in the sample this human FSH ELISA kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus FSH concentration (mIU/mL). The concentration of FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
This human FSH ELISA kit is calibrated against NIBSC/WHO, 3rd IS, HMG. In this human FSH ELISA kit, no hook effect is observed up to 5000 mIU/mL. This human FSH ELISA kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.
FSH is a hormone synthesised and secreted by gonadotropes in the anterior pituitary gland. In both males and females, FSH stimulates the maturation of germ cells. In females, FSH initiates follicular growth, specifically affecting granulosa cells. With the concomitant rise in inhibin B FSH levels then decline in the late follicular phase. This seems to be critical in selecting only the most advanced follicle to proceed to ovulation. At the end of the luteal phase, there is a slight rise in FSH that seems to be of importance to start the next ovulatory cycle. Like its partner, LH, FSH release at the pituitary gland is controlled by pulses of gonadotropin-releasing hormone (GnRH). Those pulses, in turn, are subject to the estrogen feed-back from the gonads.
The gene for the alpha subunit is located on chromosome 6p21.1-23. It is expressed in different cell types. The gene for the FSH beta subunit is located on chromosome 11p13 and is expressed in gonadotropes of the pituitary cells, controlled by GnRH, inhibited by inhibin, and enhanced by activin.
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