This Prostate-Specific Antigen (PSA) ELISA kit applies a technique called a quantitative sandwich immunoassay. This PSA ELISA kit is intended for use as a diagnostic serum marker for prostate cancer and represents a valuable new tool for the clinician. When used in combination with the digital rectal exam and/or transrectal ultrasound, PSA ELISA kit may assist in early detection programs for prostate cancer. Additionally, costly and time-consuming bone scans can be avoided for patients with newly diagnosed and untreated prostate cancer. The latter assumes a negative bone scan and therefore, a staging bone scintigram may not be necessary. Finally, an ultra-sensitive PSA assay would be useful when monitoring patients for residual disease following radical prostatectomy, as well as earlier detection of recurrent active is ease following such therapies.
Prostate-Specific Antigen (PSA) is a single-chain glycoprotein weighing approximately 34 kDa. PSA is a secretion of the prostate epithelium produced by normal, benign, and cancerous cells. PSA is functionally and immuno-chemically distinct from Prostatic Acid Phosphatase. Development of an enzyme immunoassayhas made it possible to accurately detect low concentrations of PSA in the blood of patients with malignant and benign prostate disease. PSA serum levels are elevated in patients with prostate cancer, benign prostatic hypertrophy (BPH) and inflammatory conditions associated with the surgical stage and metastasis of the disease. After several years of clinical use, PSA has emerged as the choice overall serum marker for prostate cancer. Furthermore, numerous investigators consider SA to be the most useful and meaningful tumour marker in cancer biology.
In order to measure the concentration of PSA in the sample, this human PSA ELISA kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples allowing the operator to produce a standard curve of Optical Density (O.D.) versus PSA concentration (ng/mL). The concentration of PSA in the samples is then determined using this PSA ELISA kit by comparing the O.D. of the samples to the standard curve. The microtiter plate provided in this PSA ELISA kit has been pre-coated with a monoclonal antibody specific for PSA. Standards or samples are then added to the microtiter plate wells and PSA, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of PSA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PSA are added to each well to “sandwich” the PSA immobilized on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PSA and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is easured spectrophotometrically at a wavelength of 450nm ± 2nm. This PSA ELISA kit is calibrated against NIBSC/WHO PSA First International Standard (Code 96/670). Using this PSA ELISA kit within-run coefficients of variation (cv) are 2.5 - 4.05% and between-run coefficients of variation (cv) are 4.0 - 4.5%.
Prostate specific antigen (PSA) is a protein produced by the cells of the prostate gland. PSA is present in small quantities in the serum of normal men, and is often elevated in the presence of prostate cancer and in other prostate disorders. A blood test to measure PSA is the most effective test currently available for the early detection of prostate cancer. Higher than normal levels of PSA are associated with both localized and metastatic prostate cancer (CaP).
This human PSA kit ELISA kit can be used for the quantitative determination of human Prostate-Specific Antigen (PSA) Concentrations in Serum. The estimated sensitivity for this PSA ELISA kit is to be 1 ng/mL PSA antigen in human serum. This PSA ELISA kit shows no cross-reaction and the recovery range for this PSA ELISA kit was 95 - 100%. In this PSA ELISA kit, no hook effect occurs until the concentration of PSA reaches 10,000 ng/mL. This kit is intended for LABORATORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.
Prostate specific antigen (PSA), also known as kallikrein III, seminin, semenogelase, γ-seminoprotein and P-30 antigen) is a glycoprotein manufactured almost exclusively by the prostate gland; PSA is produced for the ejaculate where it liquifies the semen and allows sperm to swim freely. It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.
Download Product Data Sheet