ALBUMIN ELISA KIT
INTENDED USE
The Albumin ELISA Kit Assay is intended for the quantitative determination of Albumin in urine and stool. For in vitro diagnostic use only.
SUMMARY AND EXPLANATION OF TEST
Albumin is the major protein in human plasma (40-60%). It is synthesized in the liver depending of the protein uptake. Albumin in fecal samples refers to an inflammatory reaction combined with intestinal bleeding. Elevated levels of albumin and hemoglobin in stool are found not only by colorectal carcinomas, but also with polyps and during chronic inflammatory diseases (Morbus Crohn or Colitis Ulcerosa).
PRINCIPLE OF THE TEST
Albumin ELISA Kit
Albumin ELISA Kit Assay is intended for the quantitative determination of Albumin in urine and stool. This Albumin ELISA Kit is a two step assay for the ultra sensitive determination of human Albumin in stool and urine. A polyclonal rabbit antibody specific for human Albumin is immobilized on microtiter plates and a second anti-Albumin antibody is conjugated to peroxidase (POD). Albumin in samples is bound to the immobilized antibody and after a washing step, to remove all unbound material. The quantification of the bound Albumin is carried out by adding a POD-labeled anti-Albumin antibody. After a second washing step a POD-substrate is added. The enzyme reaction is stopped and the absorbance is measured photometrically at 450 nm.
The intensity of the formed color is directly proportional to the concentration of Albumin in the samples.
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