Androstenedione ELISA kit can be used for the in-vitro-diagnostic quantitative determination of androstenedione in human serum and plasma. The range of the androstenedione ELISA kit is between 0 – 10 ng/ml. The analytical sensitivity the androstenedione ELISA kit was calculated from the mean minus two standard deviations of twenty (20) replicate analyses of Standard 0 and was found to be 0.019 ng/ml.
The androstenedione ELISA kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells of this androstenedione ELISA kit are coated with an antibody directed towards an antigenic site on the Androstenedione molecule. Endogenous Androstenedione of a patient sample competes with an Androstenedione horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is reverse proportional to the concentration of Androstenedione in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Androstenedione in the patient sample.
The use of control samples with this androstenedione ELISA kit is advised to assure the day to day validity of results. Use controls at both normal and pathological levels. The controls and the corresponding results of the QC-Laboratory are stated in the QC certificate added to the androstenedione ELISA kit. The values and ranges stated on the QC sheet always refer to the current kit lot and should be used for direct comparison of the results
The androstenedione ELISA kit when stored at 2-8°C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date. Opened reagents must be stored at 2-8°C. Microtiter wells must be stored at 2-8°C. Once the foil bag has been opened, care should be taken to close it tightly again.
The steroid hormone Androstenedione is one of the main androgens, besides Testosterone and Dehydroepiandrosterone. Testosterone, the most important biological active androgen, is derived from peripheral enzymatic conversion of Androstenedione. In females, the androgens are secreted mainly in the adrenal glands and in the ovary. Around 10% of the androgens are derived from peripheral conversion, mainly of DHEA. In males, androgens are secreted primarily by the Leydig cells of the testes, to some degree also in the adrenal cortex. Androstenedione and Testosterone show high diurnal variability. The highest levels are measured in the morning.
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