Aspergillus fumigatus IgM ELISA kit can be used for the qualitative and quantitative determination in human serum and plasma. The aspergillus fumigatus IgM ELISA kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The wells of the aspergillus fumigatus IgM ELISA kit are coated with antigen. Specific antibodies of the sample binding to the antigen coated wells are detected by a secondary enzyme conjugated antibody (E-Ab) specific for human IgM. After the substrate reaction the intensity of the color developed is proportional to the amount of IgM-specific antibodies detected. Results of samples can be determined directly using the standard curve.
The aspergillus fumigatus IgM ELISA kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sun light. The storage and stability of specimen and prepared reagents is stated in the instruction manual. The microtiter strips of the aspergillus fumigatus IgM ELISA kit are stable up to the expiry date of the aspergillus fumigatus IgM ELISA kit in the broken, but tightly closed bag when stored at 2–8°C.
The aspergillus fumigatus IgM ELISA kit test results are only valid if the test has been performed following the instructions. Moreover the user must strictly adhere to the rules of GLP (Good Laboratory Practice) or other applicable standards/laws. All standards must be found within the acceptable ranges as stated on the QC Certificate. If the criteria are not met, the run is not valid and should be repeated. Each laboratory should use known samples as further controls. In case of any deviation the following technical issues should be proven: Expiration dates of (prepared) reagents, storage conditions, pipettes, devices, incubation conditions and washing methods. It is recommended to participate at appropriate quality assessment trials.
The most common pathogen of the genus Aspergillus is A. fumingatus occuring in hay, grain, rotten plants and bird faeces. The main opportunistic invasive fungal infections are the candidal mycosis followed by aspergillosis. In general infections with Aspergillus spp. are airborne. Because of the ubiquity of Aspergillus species it is difficult to decide between contamination by commensalism or a serious infection. Usually infection in humans occurs in already damaged tissues only.
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