Borrelia IgG ELISA kit is an enzyme immunoassay for the in-vitro diagnostic qualitative determination of IgG antibodies against Borrelia burgdorferi in human serum und CSF. Infections with all three B. burgdorferi subspecies (garinii, afzelii and sensu strictu) are detected. Borrelia IgG ELISA kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The wells of the borrelia IgG ELISA kit are coated with antigen. Specific antibodies of the sample binding to the antigen coated wells of the borrelia IgG ELISA kit are detected by a secondary enzyme conjugated antibody (E-Ab) specific for human IgG. After the substrate reaction the intensity of the developed colour is proportional to the amount of detected IgG-specific antibodies. Results of samples can be determined directly using a Cut-off Index.
The borrelia IgG ELISA kit employs the very highly specific recombinant Borrelia burgdorferi VlsE antigen and a highly specific crude lysate antigen blend from Borrelia burgdorferi sensu strictu, B. afzelii and B. garinii and therefore determines IgG antibodies with extremely high sensitivity and specificity. The performance of the borrelia IgG ELISA kit is important especially to detect a borreliosis even in cases showing negative 14 kD + OspC titers and to monitor the immune status. The borrelia IgG ELISA kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sun light. The storage and stability of specimen and prepared reagents is stated in the corresponding chapters. The microtiter strips of the borrelia IgG ELISA kit are stable up to 3 months in the broken, but tightly closed bag when stored at 2–8°C.
Borrelia burgdorferi, a bacterium of the Spirochaetaceae, is the ethiologic agent of Lyme disease (Borreliosis) being the most common disease in Europe and the USA transmitted by tics (Ixodes sp.). Lyme borreliosis is a multi-systemic disease with a broad spectrum of clinical symptoms. A typical symptom of the acute phase is the erythema chronicum migrans (ECM), often accompanied by flue-like symptoms. In later stages of the disease arthritis, carditis, as well as neurological and dermatological manifestations may occur. Lyme borreliosis can be treated with antibiotics in all stages. Therefore, a safe and sensitive laboratory diagnosis of Lyme borreliosis, also detecting the early stage of diseases, is of major importance, since an early treatment is most appreciated. IgM antibodies usually appear approximately three weeks after the infection, IgG antibodies after four to six weeks. The early immune reaction is mainly directed against the 14 kD region of the flagellin and the OspC (Outer surface protein C). Usually the acute phase is indicated by high titers of IgM antibodies. Elevated IgG titers with low or without IgM antibodies may occur when the borreliosis is subsiding (due to therapy or spontaneously) or during the chronic stage.
The borrelia IgG ELISA kit shows high sensitivity and specificity for the detection of the Borrelia burgdorferi infection due to the use of recombinant Borrelia burgdorferi VlsE antigen combined with a highly specific mixture of antigens of Borrelia burgdorferi sensu strictu, B. afzelii and B. garinii. With this combination early stages of a Borrelia infection, and also persisting or chronic infections would be detected with high sensitivity and specificity. The borrelia IgG ELISA kit is also suitable for follow-up control of a successful therapy. In this case it has to be taken into account that antibody titers do not decrease significantly until 2 - 4 months after the infection is cured. The results themselves should not be the only reason for any therapeutical consequences. They have to be correlated to other clinical observations and diagnostic tests.
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