Brucella IgG ELISA kit is an enzyme immunoassay for the qualitative and quantitative determination in human serum and plasma. Brucella IgG ELISA kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The wells of the brucella IgG ELISA kit are coated with antigen. Specific antibodies of the sample binding to the antigen coated wells are detected by a secondary enzyme conjugated antibody (E-Ab) specific for human IgG. After the substrate reaction the intensity of the colour developed is proportional to the amount of IgG-specific antibodies detected. Results of samples using the brucella IgG ELISA kit can be determined directly using the standard curve. The brucella IgG ELISA kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sun light. The microtiter strips of the brucella IgG ELISA kit are stable up to the expiry date of the brucella IgG ELISA kit in the broken, but tightly closed bag when stored at 2–8°C.
Brucellosis is an infectious disease caused by small ellipsoid, gram-negative bacteria. There are four different germs: Br. abortus, Br. melitensis, Br. suis and Br. canis. People are infected by contact with infected animals or by swallowing meat or unpasteurized milk from infected animals. Infected humans are not contagious. The incubation period may take one to three weeks, in some cases two months. Br. Abortus and Br. melitensis cause Bang’s Disease, and raraly the Malta Fever. Typical symptoms for Bang’s Disease are periodically occuring fever, splenomegaly and swelling of lymph nodes. In some cases an inflammation of different joints and organs occurs. Malta Fever is caused by the epidemic type of brucellosis. Infection almost always leads to a manifest illness. Brucella also can cause Brucella Hepatitis. In addition, it is possible that there is a link between an infection with Brucella and the outbreak of multiple sclerosis. During an antibiotic therapy or a chronic infection, the detection of Brucella spec. in blood, urine, cerebrospinal fluid, sputum or other body fluids may be negative. Serological methods like agglutination, complement fixation reaction, Brucella Coombs test and ELISA are good alternatives. To monitor the status of infection antibodies can serve as a usual indication. During the first days, IgM is the only immunoglobulin appearing. As the disease progresses, IgM recedes quantitatively and IgG becomes predominant. In chronic brucellosis. IgG may be produced for extended periods.
The test results of the brucella IgG ELISA kit are only valid if the test has been performed following the instructions. Moreover the user must strictly adhere to the rules of GLP (Good Laboratory Practice) or other applicable standards/laws. All standards used for the brucella IgG ELISA kit must be found within the acceptable ranges as stated on the QC Certificate.
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