Chlamydia pneumoniae IgA ELISA kit is intended for the qualitative determination of IgA class antibodies against Chlamydia pneumoniae in human serum or plasma (citrate). Chlamydia pneumoniae IgA ELISA kit is a qualitative immunoenzymatic determination of IgA-class antibodies against Chlamydia pneumoniae is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells of the Chlamydia pneumoniae IgA ELISA kit are precoated with Chlamydia pneumoniae antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgA conjugate is added. This conjugate binds to the captured Chlamydia pneumoniae-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product using this Chlamydia pneumoniae IgA ELISA kit is proportional to the amount of Chlamydia pneumoniae-specific IgA antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
The reagents of this Chlamydia pneumoniae IgA ELISA kit are stable up to the expiry date stated on the label when stored at 2...8 °C. The ready to use breakapart snap-off strips of this Chlamydia pneumoniae IgA ELISA kit are coated with Chlamydia pneumoniae antigen. Store at 2...8°C. Immediately after removal of strips, the remaining strips should be resealed in the aluminium foil along with the desiccant supplied and stored at 2...8 °C; stability until expiry date.
The diagnostic specificity of this Chlamydia pneumoniae IgA ELISA kit is defined as the probability of the assay of scoring negative in the absence of the specific analyte. It is >95 %. The diagnostic sensitivity of this Chlamydia pneumoniae IgA ELISA kit is defined as the probability of the assay of scoring positive in the presence of the specific analyte. It is 88.9 %. Interferences with hemolytic, lipemic or icteric sera are not observed up to a concentration of 10 mg/ml hemoglobin, 5 mg/ml trigycerides and 0.2 mg/ml Bilirubin when this Chlamydia pneumoniae IgA ELISA kit is used.
Chlamydiae are no motile, Gram negative and obligatory intracellular growing bacteria which form characteristic inclusions within the cytoplasm of parasitized cells. They are easily visible in the light microscope. Three different Chlamydia species pathogenic for humans are known: Chlamydia trachomatis, Chlamydia pneumoniae and Chlamydia psittaci, and one species only pathogenic for animals (C. pecorum). Chlamydia trachomatis is the most prevalent agent of sexually transmitted diseases worldwide (400-500 million cases) and the number of infections is constantly growing. Pregnant women infected with C. trachomatis may transmit these bacteria during childbirth, causing conjunctivitis or pneumonia in newborns. Untreated cases of chlamydial infection can lead to chronic salpingitis, possibly resulting in ectopic pregnancy or infertility. In males, C. trachomatis is a major cause of non-gonococcal urethritis. A severe problem in Chlamydia infections is the frequent asymptomatic insidious course which may result in the initiation of chronic diseases. In many instances primary infections are not recognized and only the sequelae caused by ascended, persisting agents are diagnosed.
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