Corticosterone ELISA kit can be used for the quantitative determination of the corticosterone concentration in human serum or plasma.The corticosterone ELISA kit is based on the competition principle and the microplate separation. An unknown amount of Corticosterone present in the sample and a fixed amount of Corticosterone conjugated with horse-radish peroxidase compete for the binding sites of a polyclonal Corticosterone antiserum coated onto the wells of the corticosterone ELISA kit. After one hour incubation the microtiterplate is washed to stop the competition reaction. Having added the substrate solution the concentration of Corticosterone is inversely proportional to the optical density measured.
The minimum detectable concentration of Corticosterone using this corticosterone ELISA kit assay is estimated to be < 1.631 nmol/l. No hook effect was observed in this corticosterone ELISA kit test. Any improper handling of samples or modification of this corticosterone ELISA kit test might influence the results. Interferences caused by improper sample handling are explained in the chapters ‘Specimen - Collection’. Haemoglobin (up to 4 mg/ml), Bilirubin (up to 0.125 mg/ml) and Triglyceride (up to 30 mg/ml) have no influence on the corticosterone ELISA kit assay results.
The test results using the corticosterone ELISA kit are only valid if the test has been performed following the instructions. Moreover the user must strictly adhere to the rules of GLP (Good Laboratory Practice) or other applicable standards/laws. All standards must be found within the acceptable ranges as stated on the QC Certificate of the corticosterone ELISA kit. If the criteria are not met, the run is not valid and should be repeated. Each laboratory should use known samples as further controls. In case of any deviation the following technical issues should be proven: Expiration dates of (prepared) reagents, storage conditions, pipettes, devices, incubation conditions and washing methods. It is recommended to participate at appropriate quality assessment trials.
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