DHEA ELISA kit provides materials for the quantitative measurement of DHEA in serum. This kit is intended for in vitro diagnostic use as an aid in the evaluation of adrenal androgen synthesis. The procedure for the DHEA ELISA kit follows the basic principle of enzyme immunoassay where there is competition between in unlabelled antigen and an enzyme-labeled antigen for a fixed number of antibody binding sites. The amount of enzyme-labeled antigen bound to the antibody is inversely proportional to the concentration of the unlabelled analyte present. Unbound materials are removed by decanting and washing the wells.
When stored at 2° to 8°C unbroken reagents will retain reactivity until expiration date of this DHEA ELISA kit. Do not use reagents beyond this date. Enzyme-Conjugate, Standard Solution, Substrate Solution, Wash Solution and Zero Standard for thisDHEA ELISA kit must be stored at 2° to 8°C. Microtiter wells for thisDHEA ELISA kit must be stored at 2° to 8°C. Once the foilbag has been broken care should be taken to close it tightly again. The immuno-reactivity of the coated microtiter wells is stable for approx. 6 weeks in the broken, but tightly closed plastic zip pouch containing the desiccant. Good laboratory practice requires that controls are run with each calibration curve. A statistically significant number of controls should be assayed to establish mean values and acceptable ranges to assure proper performance of this DHEA ELISA kit.
Measurement of serum DHEA is a useful marker of adrenal androgen synthesis. Abnormally low levels may occur in hypoadrenalism, and elevated levels occur in several conditions; including virilizing adrenal adenoma and carcinoma, 21-hydroxylase and 3β-hydroxysteroid dehydrogenase deficiencies and in some cases of female hirsutism. Since very little DHEA is produced by the gonads, measurement of DHEA levels may aid in the localization of androgen source in virilizing conditions.
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