Diphtheria IgG ELISA kit is an enzyme immunoassay for the qualitative and quantitative determination in human serum and plasma. Diphtheria IgG ELISA kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The wells of this diphtheria IgG ELISA kit are coated with antigen. Specific antibodies of the sample binding to the antigen coated wells are detected by a secondary enzyme conjugated antibody (E-Ab) specific for human IgG. After the substrate reaction the intensity of the colour developed is proportional to the amount of IgG-specific antibodies detected. Results of samples can be determined directly using the standard curve.
The diphtheria IgG ELISA kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sunlight. The storage and stability of specimen and prepared reagents is stated in the protocol insert. The microtiter strips of this diphtheria IgG ELISA kit are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2–8°C.
The test results for this diphtheria IgG ELISA kit are only valid if the test has been performed following the instructions. Moreover the user must strictly adhere to the rules of GLP (Good Laboratory Practice) or other applicable standards/laws. All standards used for this diphtheria IgG ELISA kit must be found within the acceptable ranges as stated on the QC Certificate. If the criteria are not met, the run is not valid and should be repeated. Each laboratory should use known samples as further controls.
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