17-beta Estradiol ELISA kit enzyme immunoassay for the in-vitro-diagnostic quantitative determination of 17β-Estradiol in human serum and plasma. The range of this 17-beta estradiol ELISA kit assay is between 0 – 2000 pg/ml. The analytical sensitivity of this 17-beta estradiol ELISA kit was calculated from the mean minus two standard deviations of twenty (20) replicate analyses of Standard 0 and was found to be 9.714 pg/ml.
The 17-beta estradiol ELISA kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells of the 17-beta estradiol ELISA kit are coated with an antibody directed towards a unique antigenic site on the Estradiol molecule. Endogenous Estradiol of a patient sample competes with an Estradiol horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is reverse proportional to the concentration of Estradiol in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Estradiol in the patient sample.
When stored at 2-8°C unopened reagents will retain reactivity until expiration date for this 17-beta estradiol ELISA kit. Do not use reagents beyond this date. All opened reagents for this17-beta estradiol ELISA kit must be stored at 2-8°C. Microtiter wells of this 17beta estradiol ELISA kit must be stored at 2-8°C. Once the foilbag has been opened, care should be taken to close it tightly again.
Estradiol (1,3,5(10)-estratriene-3,17β-diol; 17β-estradiol; E21) is a C18 steroid hormone with a phenolic A ring. This steroid hormone has a molecular weight of 272.4. It is the most potent natural Estrogen, produced mainly by the Graffian follicle of the female ovary and the placenta, and in smaller amounts by the adrenals, and the male testes. Estradiol (E2) is secreted into the blood stream where 98% of it circulates bound to sex hormone binding globulin (SHBG) and to a lesser extent to other serum proteins such as albumin. Only a small fraction circulates as free hormone or in the conjugated form. Estrogenic activity is affected via estradiolreceptor complexes which trigger the appropriate response at the nuclear level in the target sites. These sites include the follicles, uterus, breast, vagine, urethra, hypothalamus, pituitary and to a lesser extent the liver and skin.
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