Follicle Stimulating Hormone (FSH) ELISA kit enzyme immunoassay for the in-vitro-diagnostic quantitative determination of follicle stimulating hormone in human serum. The minimal detectable concentration of human FSH by this Follicle Stimulating Hormone (FSH) ELISA kit assay is estimated to be 1 mIU/ml. No hook effect was observed in this Follicle Stimulating Hormone (FSH) ELISA kit test up to 3.000 mIU/ml of FSH in serum samples.
Follicle Stimulating Hormone (FSH) ELISA kit is a solid phase enzyme-linked immunosorbent assay based on the sandwich principle. The microtiter wells of this Follicle Stimulating Hormone (FSH) ELISA kit are coated with a monoclonal antibody directed towards a unique antigenic site on a ß-FSH molecule. An aliquot of patient serum containing endogenous FSH is incubated in the coated well with enzyme conjugate, which is an anti-FSH antiserum conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off with water. The amount of bound peroxidase is proportional to the concentration of FSH in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of FSH in the serum.
When stored at 2° to 8°C unbroken reagents will retain reactivity until expiration date of this Follicle Stimulating Hormone (FSH) ELISA kit. Do not use reagents beyond this date. Enzyme Conjugate, Substrate Solution, Standards and Zero Standard must be stored at 2° to 8°C. Microtiter wells Follicle Stimulating Hormone (FSH) ELISA kit must be stored at 2° to 8°C. Once the foilbag has been broken, care should be taken to close it tightly again. The immuno-reactivity of the coated microtiter wells Follicle Stimulating Hormone (FSH) ELISA kit is stable for approx. 6 weeks in the broken, but tightly closed bag.
Various patient serum samples of known FSH levels were mixed and assayed in duplicate. The average recovery was 98.7%. Good laboratory practice requires that controls are run with each calibration curve when using this Follicle Stimulating Hormone (FSH) ELISA kit. A statistically significant number of controls should be assayed to establish mean values and acceptable ranges to assure proper performance. Controls containing azide should not be used.
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