HCG+beta ELISA kit is an enzyme immunoassay (microtiter strips) for the quantitative determination of HCG and HCG-β in human serum. The minimum detectable concentration of beta HCG by this HCG+beta ELISA kit assay is estimated to be < 1mIU/ml. No hook effect was observed in this HCG+beta ELISA kit test up to 158.600 IU/ml of beta HCG.
The HCG+beta ELISA kitis a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells of this HCG+beta ELISA kitare coated with a monoclonal antibody directed towards a unique antigenic site on a β-hCG molecule. An aliquot of patient sample containing endogenous β-hCG or hCG is incubated in the coated well with enzyme conjugate, which is an anti-βhCG antiserum conjugated with horseradish peroxidase. After incubation, the unbound conjugate is washed off with water. The amount of bound peroxidase is proportional to the concentration of βhCG/hCG in the sample. Having added the substrate solution, theuintensity of colour developed is proportional to the concentration of βhCG/hCG in the patient sample.
When stored at 2° to 8°C unopened reagents will retain reactivity until expiration date of this HCG+beta ELISA kit. Do not use reagents beyond this date. Enzyme-Conjugate, Substrate Solution, Standards and Sample Diluent supplied with this HCG+beta ELISA kitmust be stored at 2° to 8°C. Microtiter wells of this HCG+beta ELISA kitmust be stored at 2° to 8°C. Once the foilbag has been open care should be taken to close it tightly again.
Chorionic Gonadotropin (hCG) is a glycoprotein hormone which is normally produced by the placenta during pregnancy. After conception, the hCG concentration increases rapidly to reach a peak near the end of the first trimester. High concentrations are observed throughout pregnancy. After delivery, hCG levels fall rapidly and become undetectable after a few days. Structurally intact hCG molecules are composed of an alpha and a beta subunit. The alpha subunit is nearly identical to the alpha subunits of other glycoprotein hormones, such as Thyroid Stimulating Hormone (TSH), Luteinizing Hormone (LH), and Follicle Stimulating Hormone (FSH): The differences in the beta subunit of the respective hormones account for their biological specificity and immunochemical distinctiveness. Monoclonal antibodies recognizing unique sites on the beta chain of the β-hCG/hCG molecule are essential for differentiation between hCG and LH, FSH and TSH.
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