Hepcidin Prohormone ELISA kit provides materials for the quantitative determination of hepcidin prohormone in human serum. The range of the hepcidin prohormone ELISA kit assay is between 0 – 1000 ng/ml. The analytical sensitivity for this hepcidin prohormone ELISA kit was calculated from the mean minus two standard deviations of twenty one (21) replicate analyses of Standard 0 and was found to be < 3.95 ng/ml. The analytical recovery of Pro-Hepcidin using this hepcidin prohormone ELISA kit was estimated at 3 different concentrations in serum samples. Increasing amounts of unlabeled Pro-Hepcidin (50 ng/ml, 250 ng/ml, 500 ng/ml) were added to the samples with various initial Pro-Hepcidin concentrations. Each sample (non spiked and spiked) was assayed. The Pro-Hepcidin concentrations were measured and the percentage recovery rates were calculated. This hepcidin prohormone ELISA kit assay is intended for research use only.
The hepcidin prohormone ELISA kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells of this hepcidin prohormone ELISA kit are coated with a polyclonal antibody directed towards an antigenic site on the Hepcidin Prohormone molecule (28-47 aa). Endogenous Hepcidin Prohormone of a patient sample competes with a Hepcidin Prohormone -biotin conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound biotin conjugate is reverse proportional to the concentration of Hepcidin Prohormone in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Hepcidin Prohormone in the patient sample.
When stored at 2-8°C unopened reagents will retain reactivity until expiration date for this hepcidin prohormone ELISA kit. Do not use reagents beyond this date. Opened reagents must be stored at 2-8°C. Microtiter wells of this hepcidin prohormone ELISA kit must be stored at 2-8°C. Once the foil bag has been opened, care should be taken to close it tightly again. Opened hepcidin prohormone ELISA kits retain activity for two months if stored as described above.
Hepcidin is a small cystein-rich peptide produced in the liver1. This molecule regulates the absorption of iron in the body similarly to the correlation of insulin and sugar. Hepcidin was initially isolated as a 25 amino acid (aa) peptide in human plasma and urine exhibiting antimicrobial activity 2,3. Human Hepcidin is produced from a 84 aa precursor including a putative (24 aa) signal peptide. Application of the present ELISA allows the detection and determination of Hepcidin Prohormone (Pro- Hepcidin) in serum of patients suffering from several disorders of iron metabolism.
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