Herpes-simplex-Virus (HSV) I IgM ELISA kit allows the detection of HSV 1 infection in presence of antibodies to HSV 2 in human seum. The diagnostic specificity is defined as the probability of the Herpes-simplex-Virus (HSV) I IgM ELISA kit assay of scoring negative in the absence of the specific analyte. It is >95 %. The diagnostic sensitivity is defined as the probability of the Herpes-simplex-Virus (HSV) I IgM ELISA kit assay of scoring positive in the presence of the specific analyte. It is >95 %.
Herpes-simplex-Virus (HSV) I IgM ELISA kit is a qualitative immuno- enzymatic determination of IgM-class antibodies against HSV Type 1 is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells of this Herpes-simplex-Virus (HSV) I IgM ELISA kit are precoated with HSV Type 1 recombinant antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured HSV Type 1 specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of HSV Type 1 specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader. Interferences with hemolytic, lipemic or icteric sera are not observed up to a concentration of 10 mg/ml hemoglobin, 5 mg/ml triglycerides and 0.2 mg/ml bilirubin. The reagents of this Herpes-simplex-Virus (HSV) I IgM ELISA kit are stable up to the expiry date stated on the label when stored at 2...8 °C.
Herpes simplex is an enveloped DNA virus (150-200 nm in diameter) belonging to the alpha-herpesviridae. Based on antigenic, biochemical and biologically differences it can be divided into two serotypes, HSV-1 and HSV-2. Man is the only known natural host and source of the virus. HSV Type 1 typically causes oral herpes, while HSV-type 2 typically affects the genital area. Most of the time, HSV-1 and HSV-2 are inactive, or “silent”, and cause no symptoms, but some infected people have “outbreaks” of blisters and ulcers. Once infected with HSV, people remain infected for life. Herpes simplex viruses are amongst the most common infectious agents of man, and either HSV type appears to be capable of infecting similar body sites. A high percentage of the adult population is seropositive (appr. 90% HSV-1, in dependence on the socio-economic status 10-30% HSV-2). Primary HSV-1 infection usually occurs in early childhood (6 to 18 months of age). HSV-2 usually produces mild symptoms, and most people have no recognized symptoms. Persons at risk are children with inherited T-cell deficiencies and patients who are immunosup-pressed because of infection (e.g. HIV), transplantation, or cancer therapy.
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