Luteinizing hormone (LH) ELISA kit is an enzyme immunoassay for the in-vitro-diagnostic quantitative determination of Luteinizing Hormone in human serum. The minimal detectable concentration of human luteinizing hormone by this Luteinizing hormone (LH) ELISA kit assay is estimated to be 2 mIU/ml. Various patient samples of known LH levels were mixed and assayed in duplicate. The average recovery was 101.6% when this Luteinizing hormone (LH) ELISA kit was used. Two patient samples were serially diluted with Zero Standard in a linearity study. The average recovery was 101.6% when this Luteinizing hormone (LH) ELISA kit was used. In this Luteinizing hormone (LH) ELISA kit assay, no hook effect is observed up to 4,000 mIU/ml of LH.
The Luteinizing hormone (LH) ELISA kit is a solid phase enzyme-linked immunosorbent assay based on the sandwich principle. The microtiter wells of this Luteinizing hormone (LH) ELISA kit are coated with a monoclonal antibody directed towards a unique antigenic site on a ß-LH molecule. An aliquot of patient serum containing endogenous LH is incubated in the coated well with enzyme conjugate, which is an anti-LH antiserum conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off with water. The amount of bound peroxidase is proportional to the concentration of LH in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of LH in the sample. When stored at 2° to 8°C unbroken reagents will retain reactivity until expiration date of this Luteinizing hormone (LH) ELISA kit. Do not use reagents beyond this date. Enzyme Conjugate,Substrate Solution, Standards and Zero Standard forthis Luteinizing hormone (LH) ELISA kit must be stored at 2° to 8°C. Microtiter wells must be stored at 2° to 8°C. Once the foilbag has been broken care should be taken to close it tightly again. The immuno-reactivity of the coated microtiter wells is stable for approx. 6 weeks in the broken, but tightly closed bag. Good laboratory practice requires that controls are run with each calibration curve. A statistically significant number of controls should be assayed to establish mean values and acceptable ranges to assure proper performance of this Luteinizing hormone (LH) ELISA kit. Controls containing azide should not be used.
Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormone-releasing hormone (LH-RH or Gn-RH), which is released by the hypothalamus. LH, also called interstitial cell-stimulating hormone (ICSH) in men, is a glycoprotein with a molecular weight of approximately 30.000 daltons. It is composed of two non covalently associated dissimilar amino acid chains, alpha and beta. The alpha chain is similar to that found in human thyroid-stimulating hormone (TSH), follicle stimulating hormone (FSH), and human chorionic gonadotropin (hCG). The difference between these hormones lie in the amino acid composition of their beta subunits, which account for their immunological differentiation.
Download Product Data Sheet