Measles IgM ELISA kit is an enzyme immunoassay for the qualitative and quantitative determination of IgM antibodies to measles virus in human serum and plasma. Measles IgM ELISA kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The wells of this measles IgM ELISA kit are coated with antigen. Specific antibodies of the sample binding to the antigen coated wells are detected by a secondary enzyme conjugated antibody (E-Ab) specific for human IgM. After the substrate reaction the intensity of the color developed is proportional to the amount of IgM-specific antibodies detected. Results of samples can be determined directly using single-point calibration.
The measles IgM ELISA kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sunlight. The storage and stability of specimen and prepared reagents is stated in the protocol insert. The microtiter strips of this measles IgM ELISA kit are stable up to 4 weeks in the broken, but tightly closed bag when stored at 2–8°C. The test results for this measles IgM ELISA kit are only valid if the test has been performed following the instructions. Moreover the user must strictly adhere to the rules of GLP (Good Laboratory Practice) or other applicable standards/laws. All kit controls used with this measles IgM ELISA kit must be found within the acceptable ranges as stated on the vial labels. If the criteria are not met, the run is not valid and should be repeated. Each laboratory should use known samples as further controls. In case of any deviation the following technical issues should be proven: Expiration dates of (prepared) reagents, storage conditions, pipettes, devices, incubation conditions and washing methods.
Measles is a highly contagious viral disease characterized by a clinically distinct prodrome of fever, coryza, conjunctivitis, cough and a pathognomic exanthem (Koplik`s spots). The rash of Measles appears after a 3-to 5-days prodrome, some 14 days after exposure and may be associated with edema of the skin. The disease is the result of infection with the Measles Virus, genus Morbillivirus of the family Paramyxoviridae. Complications are: otitis media, pneumonia and encephalitis. Measles have a more severe expression in younger or undernourished children with a higher incidence of hemorrhage Measles, with 5% to 10% of lethal cases.
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