ALPHA 1 MICROGLOBULIN ELISA KIT
INTENDED USE
The Alpha 1 Microglobulin ELISA Kit Assay is intended for the quantitative determination of Alpha 1 Microglobulin in urine, serum and plasma. For in vitro diagnostic use only.
SUMMARY AND EXPLANATION OF TEST
Alpha 1 Microglobulin, a glycoprotein heterogeneous in charge, was reported to occur both as a monomer of 31kDa as well as a polymer of 90kDa formed by a covalent binding with one of two alpha chains of the monomeric Immunoglobulin A.
Alpha-1-Mikroglobulin is a protein with a small molecular weight produced in the liver. In healthy persons it is metabolized in the kidneys and only minor amounts can be detected in the urine. Increased Alpha-1-Microglobulin concentration in serum is detected when the glomerular filtration rate is limited. In addition, when the ratio of total protein to the sum of albumin and a1-microglobulin is disordered, a prerenal proteinuria should be suspected.
PRINCPLE OF THE TEST
Alpha 1 Microglobulin ELISA Kit
Alpha 1 Microglobulin ELISA Kit Assay is intended for the quantitative determination of Alpha 1 Microglobulin in urine, serum and plasma. This Alpha 1 Microglobulin ELISA Kit Assay allows the quantitative determination of human alpha 1-Microglobulin from plasma, serum and urine. The alpha1-Microglobulin in the samples is bound to an excess of polyclonal rabbit anti-alpha 1-Microglobulin antibodies immobilized to the surface of the microtitre plate.
After a washing step to remove all foreign substances, the quantification of the bound alpha 1-Microglobulin is carried out by adding a peroxidase labelled antibody, which also binds to the alpha 1-Microglobulin. The amount of converted peroxidase substrate is directly proportional to the amount of bound alpha 1-Microglobulin and can be determined photometrically at 450 nm or at 405 nm if the extinction is out of range.
Download Product Data Sheet