Mycoplasma pneumoniae IgM ELISA kit is an enzyme immunoassays (microtiter strips) for the qualitative and quantitative determination of IgM antibodies against Mycoplasma pneumoniae in human serum and plasma. Mycoplasma pneumoniae IgM ELISA kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The wells of this Mycoplasma pneumoniae IgM ELISA kit are coated with antigen. Specific antibodies of the sample binding to the antigen coated wells are detected by a secondary enzyme conjugated antibody (E-Ab) specific for human IgM. After the substrate reaction the intensity of the color developed is proportional to the amount of IgM-specific antibodies detected. Results of samples can be determined directly using the standard curve.
The Mycoplasma pneumoniae IgM ELISA kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sunlight. The storage and stability of specimen and prepared reagents is stated in the protocol insert. The microtiter strips of this Mycoplasma pneumoniae IgM ELISA kit are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2–8°C. The test results using this Mycoplasma pneumoniae IgM ELISA kit are only valid if the test has been performed following the instructions. Moreover the user must strictly adhere to the rules of GLP (Good Laboratory Practice) or other applicable standards/laws. All standards used with this Mycoplasma pneumoniae IgM ELISA kit must be found within the acceptable ranges as stated on the QC Certificate. If the criteria are not met, the run is not valid and should be repeated. Each laboratory should use known samples as further controls. In case of any deviation the following technical issues should be proven: Expiration dates of (prepared) reagents, storage conditions, pipettes, devices, incubation conditions and washing methods. It is recommended to participate at appropriate quality assessment trials.
Mycoplasma pneumoniae is besides various viruses like rhinovirus, coronavirus, influenza, parainfluenza or adenovirus the reason for the classical common cold. Mycoplasms belong to the Mollicutes class. Common characteristics of the six eubacterial genera is the lack of a bacterial cell wall, osmotic fragility and small dimensions. The genome is significantly smaller compared to gram-positive and gram-negative bacteria. They depend on a host cell, respectively, on a host organism like a parasite. Mycoplasma pneumoniae is a human pathogenic bacterium causing tracheobronchitis and primary atypical pneumonia. Also secondary diseases like infarction, encephalitis, chronic neuropathy and the Guillain-Barre syndrome sometimes occur with a M.pneumoniae infection. In the laboratory, besides the cold agglutinin test and complement fixation reaction (CF), the ELISA is the method of choice showing an excellent sensitivity and the possibility to differentiate between the immunogloblin classes. Specific IgA antibodies are developed more regularly and more rapidly than IgM during an acute infection. IgA titres also decrease earlier than IgM or the late-peaking IgG response. Various studies showed that the determination of all the three immunoglobulin classes is necessary to monitor each step of the clinical
course.
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