Toxoplasma gondii IgM μ-capture ELISA kit is intended for the qualitative determination of IgM class antibodies against Toxoplasma gondii in human serum. The diagnostic specificity of this Toxoplasma gondii IgM μ-capture ELISA kit is defined as the probability of the assay of scoring negative in the absence of the specific analyte. It is 98.2%. The diagnostic sensitivity of this Toxoplasma gondii IgM μ-capture ELISA kit is defined as the probability of the assay of scoring positive in the presence of the specific analyte. It is 95.5 %. Interferences with hemolytic, lipemic or icteric sera are not observed up to a concentration of 10 mg/ml hemoglobin, 5 mg/ml triglycerides and 0.2 mg/ml bilirubin was observed for this Toxoplasma gondii IgM μ-capture ELISA kit.
The Toxoplasma gondii IgM μ-capture ELISA kit can be used for qualitative immunoenzymatic determination of IgM-class antibodies against Toxoplasma gondii is based on the ELISA (Enzymelinked Immunosorbent Assay) technique. The Toxoplasma IgM ELISA kit is an IgM-μ-capture ELISA. Microtiter strip wells of this Toxoplasma gondii IgM μ-capture ELISA kit are precoated with anti-human IgM-class antibodies to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material a complex of antigen and horseradish peroxidase labelled Toxoplasma gondii antibody is added. This complex binds to the captured Toxoplasma specific IgM antibodies. This immune complex is visualized with Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Toxoplasma gondii-specific IgM antibody in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader. The reagents of this Toxoplasma gondii IgM μ-capture ELISA kit are stable up to the expiry date stated on the label when stored at 2...8 °C.
Toxoplasma gondii is a small intracellular parasite, whose live cycle has a sexual and an asexual phase. Sexual development is restricted to the intestinal cells of (probably exclusively) cats; the oocysts formed are excreted and due to their resistant cell walls they may be infectious under advantageous circumstances for at least 1 year. Animals and man are intermediate hosts for the asexual proliferation of T. gondii: the ingested parasites will proliferate explosively within the host cells lysing them eventually. They disseminate throughout the body via circulation and lymphatic system and though may infect any cell type. In muscle and brain cells cysts are formed which are spheroidal and about 5-100 μm in diameter. Cysts are virtually immortal in the intermediate host. Toxoplasma gondii is the most common parasite in humans, but its abundance (7-80 %) is highly dependent on the geographic area, the socioeconomic status and the nutritional customs. Infection only rarely causes toxoplasmosis and usually clinical symptoms are absent, but may produce severe problems in immunosuppressed persons and fetus. Because only a primary infection during pregnancy may be dangerous and even fatal for the unborn (the probability of congenital infection is about 50%), the recent onset of an infection must be excluded. In pregnant women in over 98% of cases, the absence of IgM excludes the possibility of recent infection. In newborns the very presence of anti-toxoplasma IgM is sufficient to confirm a congenital toxoplasmosis, since maternal IgM, unlike IgG, does not cross the placental barrier. But a significant number of infected infants do not develop detectable IgM levels and thus are false negative. In immunosuppressed patients toxoplasmosis causes severe complications mostly by reactivation of an earlier latent infection.
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