Trichinella spiralis IgG ELISA kit is intended for the qualitative determination of IgG class antibodies against Trichinella spiralis in human serum. The diagnostic specificity of this Trichinella spiralis IgG ELISA kit is defined as the probability of the assay of scoring negative in the absence of the specific analyte. It is >95 %. The diagnostic sensitivity of this Trichinella spiralis IgG ELISA kit is defined as the probability of the assay of scoring positive in the presence of the specific analyte. It is >95 %. Interferences with hemolytic, lipemic or icteric sera are not observed up to a concentration of 10 mg/ml hemoglobin, 5 mg/ml triglycerides and 0.2 mg/ml bilirubin was observed using this Trichinella spiralis IgG ELISA kit.
The Trichinella spiralis IgG ELISA kit can be used for the qualitative immunoenzymatic determination of antibodies against Trichinella spiralis is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells of this Trichinella spiralis IgG ELISA kit are precoated with Trichinella spiralis ES-antigens(Excretory Secretory antigens) to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled Protein A Conjugate is added. This conjugate binds to the captured Trichinella spiralis-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Trichinella spiralis specific antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance alternative 450 nm is read using an ELISA microwell plate reader. The reagents of this Trichinella spiralis IgG ELISA kit are stable up to the expiry date stated on the label when stored at 2...8 °C.
Trichinosis (also called trichinellosis) is caused by nematodes (roundworms) of the genus Trichinella. In addition to the classical agent Trichinella spiralis, which is found worldwide in many carnivorous and omnivorous animals, four other species (T.pseudospiralis, T. nativa, T. nelsoni, and T. britovi) are recognized. Trichinosis is acquired by ingesting meat containing cysts of Trichinella. After exposure to gastric acid and pepsin, the larvae are released from the cysts and invade the small bowel mucosa where they develop into adult worms (female 2.2 mm in length, males 1.2 mm). After 1 week, the females release larvae that migrate to the striated muscles where they encyst. Encystment is completed in 4 to 5 weeks and the encysted larvae may remain viable for several years. Ingestion of the encysted larvae perpetuates the cycle. Trichinosis infection occurs worldwide, but is most common in parts of Europe and the United States. Light infections may be asymptomatic. For mild to moderate infections, most symptoms subside within a few months whereas fatigue, weakness, and diarrhoea may last for months. In severe cases, death can occur.
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