Carbon Dioxide (CO2) Assay Kit (405nm)
INTENDED USE
For the in vitro quantitative determination of Carbon Dioxide content in human serum or plasma.
CLINICAL SIGNIFICANCE
Serum CO2 is a blood test that measures the amount of carbon dioxide (CO2) in serum. Serum CO2 is really a measure of serum HCO3–, also called bicarbonate. In the body, 95% of the CO2 is present as HCO3 –, so most of what is measured in the laboratory represents HCO3 –. Higher-than-normal levels of HCO3 – may indicate excessive vomiting, respiratory dysfunction (breathing disorders), hyperaldosteronism, or Cushing syndrome. Historic procedures to measure HCO3 – in the laboratory usually involve addition of acid to liberate CO2, followed by measurement by volumetric, manometric, thermal conductivity or GC/MS, or ISE methods. These procedures are both time consuming and cumbersome. Carbon Dioxide (CO2) assay kit is a quick, easy to use enzymatic procedure applicable to routine laboratory instrumentation. The CO2 levels in the blood are influenced by kidney and respiratory (lung) function. Lower-than-normal levels of HCO3 – may indicate ketoacidosis, lactic acidosis, kidney disease, diarrhea, methanol poisoning, salicylate toxicity (such as aspirin overdose), ethylene glycol poisoning, or Addison disease (adrenal gland insufficiency).
ASSAY PRINCIPLE
Carbon Dioxide Enzymatic Assay is based on two coupled enzyme reactions including phosphoenolpyruvate carboxylase (PEPC) and malate dehydrogenase (MDH). PEPC catalyzes the first reaction which produces oxaloacetate. In the presence of MDH, the reduced cofactor is oxidized by oxaloacetate. This results in a decrease of absorbance at 405 or 415 nm that is directly proportional to CO2 concentration in the sample.
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