Potassium (K+) Ion Assay Kit
Method: Enzymatic assay
Wavelength: 450nm
Linear range: 2 – 8 mM
Intended Use
Potassium (K+) Ion assay kit can be used for the quantitative in vitro determination of Potassium in serum.
Assay Principle
Potassium is determined using Potassium (K+) Ion assay kit spectrophotometrically through a kinetic coupling assay system using potassium dependent urea amidolyase (UAL). NADH generated in a coupling enzymatic reaction reduces a water soluble colorless tetrazolium salt, WST-1 in the presence of an electron mediator, 1-methoxy-5-methyl-phenazinium methyl sulfate (PMS), to form a water soluble formazan dye, which has a maximum absorbance at 438 nm. The corresponding increase of optical density at 438 nm is proportional to the potassium concentration in the serum.