Creatinine Assay Kit
Intended Use
The Creatinine assay kit is for determination of creatinine. Creatinine is a metabolic waste product formed due to non-enzymatic dehydration of creatine derived from creatine phosphoric acid. Determination of serum or urinary creatinine is a useful diagnostic tool for kidney diseases such as acute and chronic nephritis and other disorders such as urethrophaxis, mercurialism and nephrosis.
Assay Principle
The Creatinine enzymatic assay kit for creatinine involves a series of coupled enzymatic reactions including creatininase enzymatic conversion of creatinine into the product creatine which itself is converted to sarcosine by creatine amidinohydrolase (creatinase), followed by oxidation of sarcosine by sarcosine oxidase (SOD) producing hydrogen peroxide. In the presence of peroxidase (POD) the hydrogen peroxide is quantified at 550 nm by the formation of a colored dye.
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