N-acetyl-beta-D-glucosaminidase (NAG) Assay Kit
Intended Use
The N-acetyl-beta-D-glucosaminidase (NAG) assay kit is for determination of NAG in patient urine samples. NAG is a lysosomal enzyme involved in the breakdown metabolism of glycoproteins. Increased NAG levels in urine are an early indication of renal disease and can serve as a valuable renal monitoring test in disorders such as nephritic syndrome, glomerulunephritis, drug abuse associated nephrotoxicity, diabetes-associated nephropathy, hypertension and urinary tract infections.
Assay Principle
The reagents of the assay kit are in stable liquid formulation that allows ease of use coupled with enhanced performance characteristics. NAG hydrolyses 2-methoxy-4-(2’nitrovinyl)-phenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (MNP-GlcNAc) to 2-methoxy-4-(2’-nitrovinyl)-phenol product. The product formation is detected by development of color at 505nm upon addition of alkaline buffer.
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