Source: E.Coli   Catalog No.: CHM-336   Background:

Among CXC chemokines, CXCL10 has been identified to play an important role in several endocrinological autoimmune diseases such as Hashimoto's thyroiditis, Graves'disease and Type 1 diabetes mellitus.The chemokine IP-10 (interferon-inducible protein of 10 kDa, CXCL10) is a chemoattractant for CXCR3+ T cells, binds to the G protein-coupled receptor CXCR3, which is found mainly on activated T cells and NK cells, and plays an important role in Th1-type inflammatory diseases. IP-10 also binds to glycosaminoglycans (GAGs), an interaction thought to be important for its sequestration on endothelial and other cells.

Description :

Recombinant Mouse IP-10 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8701Dalton.  Recombinant Murine IP-10 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein was lyophilized with no additives.

Solubility:

It is recommended to reconstitute the lyophilized Mouse IP-10 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized IP-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IP-10 should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).  Please avoid freeze-thaw cycles.

Purity:

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be Ile-Pro-Leu-Ala-Arg.

Dimers and aggregates:

Less than 1% as determined by silver stained SDS-PAGE gel analysis.

Biological Activity:

Murine IP-10 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract IL-2 activated T cells at 0.1-10 ng/ml.

Endotoxin:

Less than 0.1 ng/µg (IEU/µg) of Mouse IP-10.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IP-10 as a Reference Standard.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.