Source: E.Coli Catalog No.: CHM-271   Description :

Recombinant Human MCP-1 also known as Monocyte Chemotactic and Activating Factor (MCAF) produced in E.Coli is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8607 Dalton.  The rHuMCP-1 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized rHuMCP-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized rHuMCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C.   Upon reconstitution rHuMCP-1 should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).  Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Ile.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

MCP-1 is fully biologically active when compared to standard. ED50=3.0-10 ng/mL. The biological activity was determined by measuring dose dependent chemotaxis with human THP-1 cells.

Endotoxin:

Less than 0.1 ng/µg (IEU/µg) of rHuMCP-1.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a standard solution of MCP-1 as a Reference Standard.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.