Source: Submaxillary Gland of Grown Mouse   Catalog No.: CYT-440   Introduction :

NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.

Description :

NGF beta Mouse produced in Submaxillary Gland of Grown Mouse is a homodimer, non-glycosylated, polypeptide chain containing 2 identical 120 amino acids and having a molecular mass of 13,471 Dalton each.  The NGF beta Mouse is purified by advanced biology purification technology.

Synonyms:

Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.

Physical Appearance:

Sterile Filtered colorless solution at a concentration of 0.61 mg/ml.

Formulation:

The protein 0.2µm filtered solution contains 50mM PBS and 150mM NaCl.

Stability:

Nerve Growth Factor beta Mouse although stable at 14 °C for 2 weeks,  should be stored desiccated below -18 °C.  For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).  Please avoid freeze-thaw cycles.

Purity:

Greater than 99.6% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE .

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ser-Thr-His-Pro.

Biological Activity:

Activity was Measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1, the ED50 for this effect is typically 0.3 - 1 ng/ml corresponding to a Specific Activity of 1 x 106 IU/mg.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.