Source: E.Coli   Catalog No.: CYT-305   Introduction:

GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Description:

Glial derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 135 amino acids and having a total molecular mass of 30,360 Dalton.  GDNF is purified by proprietary chromatographic techniques.

Synonyms:

ATF1, ATF2, HFB1-GDNF, GDNF.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

GDNF was lyophilized after dialysis against 10mM sodium citrate and 150mM NaCl.

Solubility:

It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Glial Derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution GDNF should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).  Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.

Biological Activity:

The ED50 calculated by the dose-dependant dopamin uptake in rat mesencephalic cultures was found to be 5-10 ng/ml.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.