Source: E.Coli   Catalog No.: CYT-204   Introduction:

IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.

Description:

Interferon Alpha Human 2a Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton.  The Interferon-α 2a gene was obtained from human leukocytes.  The IFN-A 2a is purified by proprietary chromatographic techniques.

Synonyms:

Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-α 2a.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Lyophilized from (1mg/ml) solution containing 7.21 sodium chloride and 0.77 mg ammonium acetate.

Solubility:

It is recommended to reconstitute the lyophilized Interferon-alpha 2a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Interferon alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IFN-alpha 2a should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).  Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln, conforming to the sequence of native human IFN-alpha . N-terminal methionine has been completely removed enzymatically.

Biological Activity:

The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.7 x 108IU/mg.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IFN-alpha(2a) as a Reference Standard.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.