Source: E.Coli   Catalog No.: CYT-206   Introduction:

IFN-gamma produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Description :

Interferon-gamma Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 144 amino acids and having a molecular mass of 16879 Dalton. The IFN-gamma is purified by proprietary chromatographic techniques.

Synonyms:

Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein was lyophilized after extensive dialysis from a concentrated (1mg/ml) solution in 10mM sodium Phosphate buffer pH=7.4±0.1.

Solubility:

It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Interferon gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IFN-gamma should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).  Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Asp-Pro-Tyr.

Biological Activity:

The specific activity as determined in a viral resistance assay is less then 0.05 ng/ml, corresponding to a specific activity of 2.0 x 107 IU/ mg.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.640 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IFN-gamma as a Reference Standard.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.