Source: E.Coli

Catalog No.: CYT-209

Introduction :

IL-2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

Description :

Interleukin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated mutein (variant form) of human IL-2 polypeptide chain containing 134 amino acids and having a molecular mass of 15517 Dalton.
Our Interleukin -2 has an Ser substitute for Cysteine at position 126.  The IL-2 is purified by proprietary chromatographic techniques.

Synonyms:

T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein (1.1mg/ml) was lyophilized after extensive dialysis against 0.17mg sodium monobasic & 0.89mg dibasic sodium phosphate buffer to a pH=7.5.

Solubility:

It is recommended to reconstitute the lyophilized Interleukin 2 in sterile 20mM acetic acid (AcOH) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL2 should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).  Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.

Biological Activity:

The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less then 0.0645 ng/ml, corresponding to a Specific Activity of 16.9 MIU/mg.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.614 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.