Source: E.Coli

Catalog No.: CYT-328

Introduction :

IL-22 is a member of the IL-10 family of regulatory cytokines.Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes, IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the liver and pancreas. IL-22 signals through a receptor system consisting of IL-10R-beta/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family.

Description :

Interleukin-22 Human Recombinant produced in E.Coli is a single, non-glycosylated homodimeric polypeptide chain containing 2 x 146 amino acids and having a total molecular mass of 33,607 Dalton.
The IL-22 is purified by proprietary chromatographic techniques.

Synonyms:

IL-TIF, TIFa, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Each mg contains 1.6mg mannitol and 1.2mg HSA.

Solubility:

It is recommended to reconstitute the lyophilized Interleukin -22 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Interleukin 22 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL22 should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).  Please avoid freeze-thaw cycles.

Purity:

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Ile-Ser.

Biological Activity:

The Biological Acitivity was determined by the ability to activiate STAT following receptor ligand interaction.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.