Source: Insect Cells

Catalog No.: CYT-418

Introduction:

GDF8 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth.

Description:

Myostatin Human Recombinant produced in insect cells is a homodimer, non-glycosylated polypeptide chain containing 2 x 109 amino acids and having a total molecular mass of 24814 Dalton.  The GDF-8 is purified by proprietary chromatographic techniques.

Synonyms:

GDF-8, MSTN, Growth Differentiation Factor 8, MSTN Muscle Hypertrophy.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Lyophilized from a concentrated (1mg/ml) solution containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized Myostatin in sterile 20mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Myostatin although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Myostatin should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be
Asp-Phe-Gly-Leu-Asp.

Biological Activity:

The ED₅₀ as determined by the inhibition of the prolifiration of MPC-11 cells is < 20 ng/ml.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Myostatin as a Reference Standard.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.