Catalog No.: ENZ-275   Description:

The Enzyme (29 KD) Aeromonas Aminopeptidase is used in the processing of pharmaceutical proteins produces by genetic engineering as well as for physical and structural investigations and for sequence and amino-terminal determinations. This exopeptidase recognizes a specific stop sign at –X- Pro and requires a free a-amino group in the L-configuration. It is therefore suitable for the removal of the redundant N-terminal methionine often added to engineered recombinant proteins.

Synonyms:

Bacterial leucyl aminopeptidase, EC 3.4.11.10.

Physical Appearance:

Sterile filtered liquid formulation.

Formulation:

Buffered solution containing 4.16 mg/ml in 10mM Tris-HCL, 100mM NaCl, 5µM ZnSO4, pH 8.0.

Stability:

Two years when stored at –20 °C, 2 weeks at 4°C. Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Unit definition:

120 Units/mg protein. One unit is defined as the amount of enzyme that hydrolyses 1µmole of L-leucine p-nitroanilide at 25 °C per 1 minute.

Biological Activity:

Recombinant Aeromonas Aminopeptidase was found to have an activity of 120 Units/mg protein.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.