Source: Chinese Hamster Ovary Cells   Catalog No.: ENZ-319   Introduction:

Deoxyribonuclease I Human Recombinant (rhDNase), an enzyme which selectively cleaves DNA. Recombinant Human Dnase is an endonuclease enzyme which splits phosphodiester linkages within polynucleotides, acting primarely on single stranded DNA (ssDNA), double stranded DNA (ddDNA) and chromatin. Dnase is activated by bivalent metals such as Mg+2 and Ca+2.  Dnase enzymes are common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. Dnase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membrane-bound DNA fragments.

Description:

Deoxyribonuclease I Human Recombinant produced in CHO is a glycosylated, polypeptide chain containing 260 amino acids and having a total molecular mass of 37,000 Dalton with a molecular formula of C1321H1995O396S9.  DNase is purified by proprietary chromatographic techniques.

Synonyms:

EC 3.1.21.1, Deoxyribonuclease I, DNase I, Dornase alfa, DNL1, DRNI, FLJ38093.

Physical Appearance:

Sterile liquid colorless solution at a concentration of 1 mg/ml.

Formulation:

Each mg contains 150 µg calcium chloride dihydrate and 8.77 mg NaCl.

Stability:

2 years when stored at 4°C, three weeks at 15°C, pH-6.3.

Unit Defenition:

Dnase is generally assayed according to the photometric method developed by Kunitz (see Kunitz, M., Journal of General Physiology, vol 33, p.349 and 363, 1950). One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH-5. Also 0.005 Kunitz unit digests 1µg of lambda phage DNA in 10 minutes at 37°C in50mM Tris, 1mM Mg++, pH 7.8 in a 50ul reaction.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.