Source: Bacillus sphaericus   Catalog No.: ENZ-269   Description:

Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination.

Synonyms:

Lysostaphin, EC 3.4.24.75, Glycyl-glycine endopeptidase.

Physical Appearance:

Sterile Filtered lyophilized powder.

Formulation:

The protein contains 20mM sodium acetate, pH 4.5.

Stability:

Lysostaphin although stable at 4°C for 6 months, should be stored desiccated below -18°C.  Please avoid freeze-thaw cycles.

Solubility:

It is recommended to reconstitute the lyophilized Lysostaphin in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Purity:

Greater than 93.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Biological Activity:

Determined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH-8.0, 30°C. Specific activity is measured with a defined activity of 4,000 U/mg.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis RP-HPLC, using a calibrated solution of Lysostaphin as a Reference Standard.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.