Source: E.Coli   Catalog No.: ENZ-271   Introduction:

Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28 kDa proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18 kDa active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.

Description:

Matrix Metalloproteinase-7 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 19.13 kDa.  The MMP-7 is purified by proprietary chromatographic techniques.

Synonyms:

Matrilysin, EC 3.4.24.23, Pump-1 protease, Uterine metalloproteinase, Matrix metalloproteinase-7, MMP-7, Matrin, MPSL1, PUMP-1.

Physical Appearance:

Sterile filtered clear solution.

Formulation:

The protein (1.9 mg/ml) contains the following additives 10mM HEPES (pH 7.4), 5mM CaCl2 and 150mM NaCl.

Stability:

Matrilysin, although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.  Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Biological Activity:

The specific activity was found to be 3100 IU/mg.

Unit definition:

One unit is defined as the digestion of 1 µg Azocoll/min at 37°C.

Applications:

1.Western Blotting (1:1K-5K for neat serum and 1-10 ug/ml for affinity pure antibody using ECL technique). 2.ELISA: Control peptide can be used to coat ELISA plates at 1 ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1 ug/ml for affinity pure). 3.Histochemistry and Immunofluorescence: Not tested.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.