Source: E.Coli   Catalog No.: ENZ-301   Introduction:

Full-length recombinant human neutrophil pro-collagenase (MMP-8), latent form. Matrix metalloproteinase 8 (MMP-8), or neutrophil collagenase, degrades interstitial collagens, acting preferentially on collagen type I. Increased full-length MMP-8 protein was associated with infiltration into the skin of neutrophils, which are the major cell type that expresses MMP-8.  MMP-8 is synthesized and stored in specific granules in neutrophil leukocytes. MMP-8 activity is therefore regulated by factors such as surface-bound ligands (IgG or complement components) that release it through degranulation.Once released and activated through proteolytic or oxidative mechanisms, MMP-8 plays a major role in the connective tissue turnover that accompanies inflammatory processes.

Description:

Matrix Metalloproteinase-8 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 75 kDa.  The MMP-8 is purified by proprietary chromatographic techniques.

Synonyms:

Neutrophil collagenase, EC 3.4.24.34, Matrix metalloproteinase-8, MMP-8, PMNL collagenase, PMNL-CL, HNC, CLG1.

Physical Appearance:

Sterile Filtered clear solution.

Formulation:

The protein Solution (100 µg/ml, 0.1 ml) in 0.05M Tris-HCl buffer, pH 7.5, containing 0.2M NaCl, 5mM CaCl2, 20µM ZnSO4 and 0.1% BSA.

Stability:

MMP-8 although stable at 140C for 1 week, should be stored desiccated below -180C.  Please avoid freeze-thaw cycles.

Purity:

Greater than 90% as determined by SDS-PAGE.

Unit definition:

One unit of collagenolytic activity is defined as the cleavage of 1µg of collagen per minute.

Biological Activity:

Activity was found to be 100 units/ml after activation with APMA by solution assay method (10U=100 assays).

Applications:

Used as a standard for analyzing mammalian collagenase activity.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.