Source: E.Coli   Catalog No.: ENZ-288   Description :

Recombinant Staphylokinase produced in E.Coli is a non-glycosylated polypeptide chain having a molecular weight of 15.5kd.  Staphylokinase is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Recombinant SAK was lyophilized from a sterile solution containing 20mM phosphate buffer pH-7.

Solubility:

It is recommended to reconstitute the lyophilized SAK in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized SAK although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution SAK should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).  Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Biological Activity:

The biological activity measured by the ability of fibrin lysis in agarose plate was found to be 4600IU/mg.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ser-Ser-Phe-Asp.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/µg (IEU/µg) of Staphylokinase.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Staphylokinase as a Reference Standard.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.