Source: Sf9 insect cells. Catalog No.: ENZ-306

Description :

DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis. In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100 kDa molecular weight. Most of this precursor is then proteolytically processed to a species with 70 kDa molecular weight from which the Scl-70 antigen has derived its name. Recombinant Human Scl-70 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 dalton. Recombinant Scl-70 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human Scl-70 is supplied at a of 0.9mg/ml in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% glycerol.

Stability:

Store at 4°C if entire vial will be used within 2-4 weeks.  Store, frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.

Immunological functions:

1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Applications:

Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

Coating concentration:

0.5-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.

Purity:

Greater than 80% (SDS-PAGE).

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.