Source: CHO   Catalog No.: ENZ-263   Description :

Recombinant Human Tissue Plasminogen Activator produced in Chinese Hamster Ovary Cells (CHO) is a single, glycosylated polypeptide chain containing 527 amino acids and having a molecular mass of 59008.71 Dalton.  Human TPA is a serine protease enzyme that converts plasminogen to plasmin.  Human Tissue Plasminogen Activator is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Each mg of Human Tissue Plasminogen Activator contains 1.7gr L-arginine, 0.5gr phosphoric acid and 4mg tween 80.

Solubility:

It is recommended to reconstitute the lyophilized Human TPA in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Human TPA although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Human TPA should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).  Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be :Ser Tyr Gln Val Ile .

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Enzymatic Activity:

Human TPA is fully biologically active when compared to World Health Organization (WHO) reference standard which is 5.8x105 IU/mg.

Endotoxin:

Less than 0.1 ng/µg (IEU/µg) of Recombinant Tissue Plasminogen Activator.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Tissue Plasminogen Activator as a Reference Standard.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.