Source: Chinese Hamster Ovary Cells   Catalog No.: CYT-276   Introduction :

BMP-7 (osteogenic protein 1) belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.

Description :

N-TERMINAL---Human BMP-2 (Met 1 – Arg 282) Human BMP-7 (Ser 293 – Arg 431)---C-TERMINAL. The DNA sequence encoding the human BMP-2 signal peptide and propeptide (1~282 amino acid) fused to the human BMP-7 mature chain (293~431 amino acid) was expressed in a Chinese hamster ovary cell line. The mature recombinant BMP-7 generated by the proteolytic removal of the signal peptide and propetide contains 139 amino acid residues. The glycosylation of BMP-7 increases the molecular mass and the glycosylated proteins migrate as 25 ~ 40 kDa in SDS-PAGE under non-reducing conditions.  BMP-7 is purified by proprietary chromatographic techniques.

Synonyms:

Osteogenic Protein 1, OP-1, BMP-7.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 1% sucrose, 1.2% mannitol, 20mM glycine, and 0.005% tween 20 pH-4.

Solubility:

It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-7 in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution BMP 7 Human should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).  Please avoid freeze-thaw cycles.

Purity:

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b Analysis by SDS-PAGE.

Biological Activity:

Measured in alkaline phosphatase activity assay using MC3T3-E1 cells. The ED50 for this effect is < 100 ng/mL.

Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.