ANTI-heTG IgA ELISA KIT
INTENDED USE
The Anti-heTG IgA ELISA Kit Assay is intended for the quantitative determination of IgA anti-epidermal transglutaminase antibodies in serum and plasma. It is for in vitro diagnostic use only.
INTRODUCTION
Dieterich et al. have shown, that tissue transglutaminase (tTG) is the predominant endomysial auto antigen characteristic for celiac disease.
In most celiac patients, usually several disorders are observed, e.g. malabsorption, infertility, osteoporosis and delayed growth of children. But it has also been widely reported that celiac disease is associated with a whole series of autoimmune diseases like Dermatitis herpetiformis Duhring, Diabetes mellitus, rheumatoid Arthritis, IgA-nephritis, neuro-psychiatric disorders, Hashimoto-Thyreoditis / M. Basedow and an increased risk of developing malignant T cell lymphoma.
Because the prevalence of associated autoimmune diseases in most cases is high, it is advisable to determine the auto antibodies against tissue transglutaminase (tTG) as a marker for celiac disease.
PRINCIPLE OF THE TEST
Anti- heTG IgA ELISA Kit
Anti-heTG IgA ELISA Kit Assay is intended for the quantitative determination of IgA anti epidermal-transglutaminase antibodies in serum and plasma. This Anti- heTG IgA ELISA Kit Assay is a sandwich assay for the quantitative determination of anti-epidermal transglutaminase antibodies (IgA anti-heTG).
The wells of the microtiter plate are coated with the antigen. In a first incubation step, the anti-heTG antibodies are bound to the coated antigen. To remove all unbound substances, a washing step is carried out. In a further incubation step, a peroxidase-labeled antibody is added. After another washing step to remove all unbound substances, the solid phase is incubated with the peroxidase substrate, tetramethylbenzidine (TMB). Finally, an acidic stop solution is added to terminate the enzymatic reaction, whereby the colour changes from blue to yellow. The intensity of the yellow colour is directly proportional to the IgA anti-epidermal-transglutaminase antibody concentration. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standards. IgA anti-epidermal-transglutaminase antibodies present in the samples are determined directly from this curve.
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