EOSINOPHIL DERIVED NEUROTOXIN (EDN) ELISA KIT
INTENDED USE
The Eosinophil Derived Neurotoxin (EDN) ELISA Kit is intended for the quantitative determination of EDN (eosinophil-derived neurotoxin, eosinophil protein x, EPX) in serum, plasma, urine and stool. It is for in vitro diagnostic use only.
INTRODUCTION
EDN (eosinophil-derived neurotoxin, eosinophil protein x, EPX), a cationic glycoprotein, which is released by activated eosinophiles, has strong cytotoxic characteristics and plays a significant role in the prevention of virus infections. It is released by the eosinophile granules in places where eosinophiles are mainly found: in the skin, lungs, urogenital and gastrointestinal tract, that is, in the organs acting as an entry point for pathogens. The accumulation of EDN in the intestine is associated with inflammation and tissue damage. Measuring of EDN in stool can serve as an objective parameter for a current clinical or sub-clinical chronic inflammation located in the gastrointestinal area. In the case of Colitis ulcerosa and Crohn’s disease, the EDN measurement enables the evaluation of a disease’s activity and the prediction of a relapse.
PRINCIPLE OF THE TEST
Eosinophil Derived Neurotoxin (EDN) ELISA Kit
The Eosinophil Derived Neurotoxin (EDN) ELISA Kit is intended for the quantitative determination of EDN (eosinophil-derived neurotoxin, eosinophil protein x, EPX) in serum, plasma, urine and stool. The Eosinophil Derived Neurotoxin (EDN) ELISA Kit assay utilizes the two-site “sandwich” technique with two selected antibodies (monoclonal and polyclonal) that bind to human EDN.
Assay standards, controls and prediluted patient samples containing human EDN are added to wells of microplate that was coated with a high affine monoclonal anti-human EDN antibody. After the first incubation period, antibody immobilized on the wall of microtiter wells captures human EDN in the sample. Then a peroxidase-conjugated rabbit polyclonal anti-human EDN antibody is added to each microtiter well and a “sandwich” of capture antibody - human EDN – Peroxidase-conjugate is formed. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of EDN. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. EDN present in the patient samples, is determined directly from this curve.
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