GST-Pi ELISA KIT
INTENDED USE
The GST-Phi ELIS Kit Assay is intended for the quantitative determination of GST-Phi in serum and tumor tissue extract. For in vitro diagnostic use only.
INTRODUCTION
Glutathion-S-transferases (GST) are a group of enzymes, which are involved in detoxification processes. The GSTs are divided in 5 subgroups: Alpha, Mu, Pi, Teta and microsomal. The classes differ in their physicochemical, immunological, enzymatic and structural properties. Their function in cellular metabolism is the coupling of electrophile compounds, for example most of the carcinogens, to Glutathion (GSH). The GST Pi is the main group in erythrocytes and in some leucocytes, moreover they are found in most tissues except liver. The GST Pi are dimeric molecules with a molecular weight of 46000 Da and an isoelectric point of about 4.7. High GST Pi levels were found in all neoplastic tissue (fetal, maligne) and in serum of patients with post-operative relapse. Increased serum levels were also found in haematological diseases (haemolytic anaemia and sub-groups of leukemia). Moreover GST Pi concentrations give information about the multi-drugresistance-status of tumours.
PRINCIPLE OF THE TEST
GST-Phi ELISA Kit
GST-Phi ELIS Kit Assay is intended for the quantitative determination of GST-Phi in serum and tumor tissue extract. This GST-Phi (EIA) allows the quantitative determination of human GST Pi. GST Pi is first coated to the surface of the microtiter plates. After a blocking step and a preincubation of calibrators and samples with a polyclonal rabbit antibody the GST Pi in the controls and samples compete with the GST Pi on the plate for the antibody binding. After a washing step the detection of the bound rabbit antibody is performed by a peroxidase labeled goat anti rabbit antibody (POD-antibody). The amount of converted substrate (TMB) is indirectly proportional to the amount of GST Pi antigen in the sample and can be determined photometrically at 450 nm.
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