BETA DEFENSIN 2 ELISA KIT
INTENDED USE
This Beta Defensin 2 ELISA Kit is intended for the quantitative determination of beta-Defensin 2 in stool. For in vitro diagnostic use only.
SUMMARY AND EXPLANATION OF THE TEST
The ß-defensins are an integral part of the congenital immune system and contribute with their antimicrobial effect to the barrier function of intestinal epithelial cells.
Defensins exert a variable degree of antimicrobial activity against bacteria, fungi, and some enveloped viruses. Vertebrate defensins are classified as alpha- or beta-defensins, based on their pattern of disulfide bridges. Nine human defensins of epithelial origin have been found, three of them being ß-defensins (HBD-1, -2 and -3). The expression of ß-defensins is induced by the pro-inflammatory cytokines and also through microorganisms (e.g. E.coli, H. pylori or P. aeruginosa).
A ß-defensin-2-deficiency can, for example, be observed in the intestinal mucous of patients with Crohn’s disease. The defense system of the mucous membrane is therefore restricted and allows an increased invasion of bacteria, which could possibly lead to a typical infection in Crohn’s disease patients.
Whether the ß-defensin-2 deficiency could even play a role in the development of Crohn’s disease is currently being researched. As is the possibility that it is the probiotic bacterium, which produces ß-defensin.
PRINCIPLE OF THE TEST
Beta Defensin 2 ELISA Kit
This Beta Defensin 2 ELISA Kit is intended for the quantitative determination of beta-Defensin 2 in stool. The beta-defensin 2 in standard and samples is bound to an available excess of polyclonal antibodies against beta-defensin 2, which are immobilized on the surface of the microtiter plate. After a washing step, to remove all interfering substances, the quantification of bound beta-defensin 2 is carried out by adding a polyclonal anti beta-defensin 2 antibody, which is biotinylated. This antibody is detected with horseradish peroxidase labeled streptavidin. The amount of converted substrate by Peroxidase is directly proportional to the amount of bound beta-defensin 2 and can be determined photometrically at 450 nm.
Download Product Data Sheet