THYMOSIN BETA 4 ELISA KIT
INTENDED USE
The Thymosin Beat 4 ELISA Kit Assay is intended for the quantitative determination of Thymosin Beat 4 in serum and thymus extract. It is for research use only.
INTRODUCTION
The beta-thymosins are a family of highly conserved polar 5 kDa peptides originally thought to be thymus hormones. Further studies demonstrated that beta-thymosins are ubiquitous; they have been found in a variety of tissues and cell lines. The highest concentrations have been detected in spleen, thymus, lung, and peritoneal macrophages.
Thymosin Beta 4 binds monomeric actin in a 1:1 complex acting as an actin buffer, preventing polymerization into actin filaments but supplying a pool of actin monomers when needed by the cell. Changes in the expression of Thymosin Beta 4 appear to be related to the differentiation of cells. Thymosin Beta 4 is detected outside of cells in blood plasma and wound fluid. Several biological effects are attributed to Thymosin Beta 4, like induction of metallo-proteinases, chemotaxis, angiogenesis and inhibition of inflammation as well as inhibition of bone marrow stem cell proliferation.
PRINCIPLE OF THE TEST
Thymosin Beat 4 ELISA Kit
Thymosin Beat 4 ELISA Kit Assay is intended for the quantitative determination of Thymosin Beat 4 in serum and thymus extract. This Thymosin Beat 4 ELISA Kit can be used for the determination of Thymosin Beta 4 (T Beta 4) in serum and thymus preparations.
The test principle is based on a competition between antigen in the sample or standards and the antigen coated on the wells of microplate. A peroxidase-conjugated antibody is used for detection and quantification, and tetramethylbenzidine (TMB) as a peroxidase substrate. The enzymatic reaction is terminated by acidic stop solution. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from standard. T Beta 4 present in the patient samples is determined directly from this curve.
Download Product Data Sheet